Not known Facts About attune flow cytometer
Not known Facts About attune flow cytometer
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At that time, the early indications were dismissed as particles, however the retrospective analysis demonstrated shared characteristics with the problematic cells in lifestyle. Tracing the an infection aided the lab establish further laboratory procedures for screening and protection of assay-critical cell lines.
As samples are acquired on the Attune CytPix Flow Cytometer, the large-speed brightfield digicam captures and outlets pictures of detected occasions at a charge approximately six,000 images per 2nd, depending on the flow level and picture measurement. For higher overall flexibility, Attune Cytometric Application enables you to alter the image seize frequency as needed and choose a specific gate for image seize, if essential.
Combine acoustic focusing technology with superior-parameter Examination for flexible spectral unmixing and standard workflows.
The award-winning Invitrogen Attune CytPix Flow Cytometer combines acoustic concentrating which has a higher-pace brightfield digicam for simultaneous high throughput flow cytometry and high resolution brightfield imaging.
*Pricing is indicative only and is topic to alter. Remember to contact your Agilent consultant with any inquiries. The information set forth herein just isn't a proposal or possibly a motivation, each of which might be issue to credit history acceptance, terms and conditions, and availability with Agilent. Agilent Fiscal Alternatives is accessible in selected international locations.
Here, a seasoned user has gated singlets confidently. Just after assessing the guide singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate is made up of in excess of 4% aggregates.
Pair your Attune flow cytometer using an autosampler for elevated workflow performance, and include robotic integration for optimum throughput.
Dependable graphic good quality even at higher throughput Acoustic focusing plus a high-pace digital camera combine to impression these T cells persistently at low or higher flow costs. Simply alter concentrate and digicam options to fulfill experimental needs.
The Attune NxT cytometer may be the reliable workhorse instrument your lab can count on for consistent and dependable effectiveness. The Attune CytPix product features a significant-velocity brightfield camera and AI-pushed graphic Examination program, with the latest upgrades including machine Mastering applications and an intuitive consumer interface, enabling Improved facts precision, precision, and a lot quicker insights into sample biology.
Potentially most of all, these gatherings have cells of Evidently different phenotypes which may lead to incorrect conclusions with regards to double positive functions (specifically in rare populations).
AI-driven automated image Evaluation While using the Attune Cytometric Computer software translates celebration features into unique morphology parameters which can be combined with common fluorescence and scatter parameters to expose very reproducible populace improvements and unique subsets.
We Formerly demonstrated the power of imaging Car or truck-T/Ramos cell interactions. Allow’s have a look at just the populace of greatest fascination, the double favourable activities, to learn more. We could now use extended morphological parameters (here, circularity and skewness of depth) to additional analyze the features of these populations and refine gating on these events, growing data robustness.
Developed-in dimensionality reduction equipment increased resolution into populations. Fluorescently gated populations back-gated on to UMAP plot to substantiate predicted inhabitants resolution in lysed full human blood. Person can view activities from each cluster for Visible affirmation of mobile phenotypes.
Adaptable payment program from Agilent Financial Options assist you to have the significant instrumentation you'll need for speedy challenge, or to develop your lab.
Gating on these extended parameters permits you to swiftly and correctly detect populations of fascination and ensure gating technique with little or no handbook overview. The Attune application presents use of point out-of-the-artwork machine Discovering Evaluation resources within an expertly developed consumer interface, which makes it exceptionally user friendly.
The Attune Xenith cytometer is usually a superior-parameter spectral analyzer, providing the two spectral unmixing and standard flow abilities for check here detailed and flexible mobile Assessment.
Agilent Price Assure guarantees you not less than 10 years of instrument use from the day you purchase. In any other case, We are going to credit you Together with the residual value of the procedure toward an upgraded model.
Below, we present that by gating methods using image-primarily based quantitative parameters can distinguish interacting cells from coincident events to extra precisely review interacting cells.