The Basic Principles Of flow cytometer
The Basic Principles Of flow cytometer
Blog Article
Facts-enriched characterization of mobile populations. Morphological facts from photographs can add to the richness of flow cytometry details. For instance, the determine displays an normally traditional apoptosis assay using Annexin V and PI, adding mobile imaging to characterize cells in Just about every population to expose morphologically distinctive capabilities. These insights could not are received from multiplex staining alone.
As samples are obtained within the Attune CytPix Flow Cytometer, the high-velocity brightfield camera captures and shops photos of detected functions in a fee up to 6,000 pictures for each next, depending upon the flow price and picture dimension. For bigger adaptability, Attune Cytometric Program lets you modify the image capture frequency as essential and choose a particular gate for picture capture, if essential.
Incorporate acoustic focusing technologies with large-parameter analysis for adaptable spectral unmixing and conventional workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing having a higher-velocity brightfield digicam for simultaneous higher throughput flow cytometry and substantial resolution brightfield imaging.
"An image isn't really fairly truly worth one thousand dots, but it really actually helps you to see photographs. It is great to check out pictures that correlate with staining. One example is, AnnexinV-beneficial cells which are morphologically different than healthier cells, or CD14+ monocytes that happen to be greater and more textured than CD3+/CD4+/CD14- lymphocytes.
Also, it can be illuminating to determine what number of doublets slip via conventional singlet gates, and fantastic to verify the position of unexpected double-constructive occasions in my Investigation."
Optimizing gating tactic. Even sturdy guide singlet gating is error-prone and stays a subjective decision issue in Virtually all flow cytometry assays. Imaging may be used to confirm and adjust gates to incorporate only solitary cells of fascination.
Steady impression top quality even at higher throughput Acoustic focusing and a substantial-speed digital camera Incorporate to image these T cells continually at minimal or superior flow charges. Simply regulate target and camera options to fulfill experimental necessities.
The Attune NxT cytometer is the dependable workhorse instrument your lab can rely on for reliable and dependable effectiveness. The Attune CytPix design contains a large-velocity brightfield camera and AI-driven graphic Investigation program, with recent upgrades together with equipment Discovering equipment and an intuitive person interface, enabling enhanced information precision, precision, and more rapidly insights into sample biology.
People can check out occasion images in genuine-time to confirm sample excellent, optimize protocols, and history the morphology of each mobile populace determined for analysis. Imaging capture also Positive aspects from acoustic focusing, which helps you to position the cells to ensure a sharp, centered image is obtained.
Seem waves help precise cell alignment, resulting in Excellent data top quality and ~10X a lot quicker sample flow premiums.
We Beforehand demonstrated the strength of imaging CAR-T/Ramos cell interactions. Enable’s evaluate just the population of biggest desire, the double good gatherings, to learn more. We can now use prolonged morphological parameters (here, circularity and skewness of intensity) to further more look at the functions of those populations and refine gating on these activities, expanding details robustness.
Leverage the strength of Highly developed technologies and innovation to accelerate your discoveries. Have faith in in the Attune Flow Cytometer family to provide the trustworthiness, speed, and precision that set your operate apart.
No clean/no lyse leukocyte analysis with imaging. Cells ended up acquired from 24-hour-aged blood by dilution in one mM EDTA ( Professional UX/UI enables people to prepare AI designs for expanded apps. (A) The “Train Model” attribute presents annotation applications and serious-time design education precision.
At the heart of each Attune Flow Cytometer lies our innovative acoustic focusing technologies, which utilizes seem waves to meticulously align cells inside the read more flow cell. This innovative approach surpasses classic hydrodynamic solutions, resulting in speedier processing speeds, Extraordinary facts quality, and amplified sample throughput.
The Attune Xenith cytometer can be a superior-parameter spectral analyzer, giving the two spectral unmixing and standard flow abilities for detailed and adaptable cell Examination.
Agilent Worth Guarantee ensures you at the least ten years of instrument use within the date you buy. Or else, We are going to credit history you Together with the residual price of the process toward an upgraded product.
In this article, we present that by gating techniques using picture-based quantitative parameters can distinguish interacting cells from coincident occasions to more properly examine interacting cells.